Fig. 2

NMD is hyperactivated during mouse cerebellum and hippocampus development in the Fmr1-KO mouse, and FMRP co-immunoprecipitates with UPF1 and p-UPF1 in P0 cortex of the WT mouse. a As in Fig. 1d, but for WT and Fmr1-KO cerebellum. Representative of three independently performed experiments. b As in Fig. 1f, but for WT and Fmr1-KO cerebellum. Means ± S.D., where n = 3 (WT) and 3 (Fmr1-KO). c As in Fig. 1 g, but for WT and Fmr1-KO cerebellum. Means ± S.D., where n = 3 (WT) or 3 (Fmr1-KO). (*)P < 0.05 compares Fmr1-KO cells relative to WT cells (two-sided unpaired t test). d As in a, but for the hippocampus. e As in b, but for the hippocampus. f As in c, but for the hippocampus. Means ± S.D., where n = 3 (WT) and 3 (Fmr1-KO). (*)P < 0.05 compares Fmr1-KO cells relative to WT cells (two-sided unpaired t test). g Western blots of lysates of WT P0 cortex before (−) or after IP with (+) or without (−) RNase I using anti(α)-UPF1 or, as a negative control, normal rabbit serum (NRS). Representative of two independently performed experiments. h As in g, but using α-p-UPF1 and, as a negative control rabbit IgG (rIgG) in IPs. Representative of two independently performed experiments