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Fig. 3 | Genome Biology

Fig. 3

From: Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing

Fig. 3

Summary of differential transcript usage results from FLAMES. A Summary of results from the statistical testing of DTU detected many significant genes per sample (adjusted P-value < 0.01). B Table of common functional categories among different samples from the functional enrichment analysis of gDTU. C Top 4 most abundant isoforms of RPS24 in human and heatmap of their expression at the single-cell level in the scmixology1, scmixology2, and CLL2 samples. D Top 4 most abundant isoforms of RPS24 in mouse and heatmap of their expression at the single-cell level in MuSCs. E UMAP of cells in CLL2, colored by RPS24 gene expression and transcript expression. Two transcripts with differential expression on different populations were selected. Transcript expression in each cell is colored by scaled relative expression to highlight the difference between different populations. F Similar to E, UMAP of cells in MuSC sample, colored by RPS24 gene expression and transcript expression. G Top 4 most abundant isoforms of CD45 in CLL2 and UMAP visualizations of the cells colored by (from left to right) gene expression, transcript expression, and corresponding protein expression. H Top 4 most abundant isoforms of CD82 in MuSC, with UMAP visualization of cells colored by expression of two isoforms that have differential expression between quiescent and activated MuSC. I scATAC-seq read coverage for PRDX1 with cells from each cell line aggregated and plotted together. UMAP plots showing isoform expression, with each cell colored by scaled transcript expression

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