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Fig. 2 | Genome Biology

Fig. 2

From: Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing

Fig. 2

Overview of the single-cell isoform-level analysis from FLAMES. A Classification of transcripts according to their splice sites when compared to reference annotations. B Summary of transcripts in different categories in A both in numbers (left) and in the percentage of UMI counts (right). C UpSet plot showing overlap of transcripts in human datasets, where the number of transcripts shared by different sets of samples is indicated in the top bar chart, colored by categories specified in A. D UMAP visualization of CLL2 and MuSC dataset on the cells sampled for long-read sequencing. Colored by percentage of UMI counts of transcripts in FSM (top) and NNC (bottom) categories. CLL cells and quiescent MuSCs are annotated on the plot. E Bar plot of the number of distinct transcripts expressed per gene. Genes with more than five distinct transcripts are merged. F Box plot showing the percentage of transcript abundance relative to gene abundance for genes express multiple transcripts. Transcripts are ranked by abundance, shown on the x-axis. G Summary of the type of alternative splicing between the two most abundant transcripts of each gene. The “Complex splicing changes” category represents transcripts with more than one type of constitutive alternatively spliced event

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