Fig. 5

Identification of the candidate gene MeAHL17 associated with cassava bacterial blight (CBB). a Manhattan plots for CBB resistance on chromosome 2 using FAST-LMM. b Local manhattan plot (top) and linkage disequilibrium heat map (bottom). Red dashed lines indicate the candidate region. Blue arrow indicates the SNP in the candidate gene. c Comparison of CBB resistance based on the SNPs of AA and AG. The symbol n represents the number of accessions with the same genotype. d Differences in expression of MeAHL17 between CBB-susceptible accessions (carrying AA allele) and CBB-resistant accessions (carrying AG allele) at (0 days post inoculation (dpi)) and 2 dpi of Xam infection, as detected by qRT-PCR (**P < 0.01; two-tailed t-test). The symbol n represents the number of accessions. Each accession contains three biological replicates. The center line represents the median, box limits indicate the upper and lower quartiles, and whiskers denote the range of the data. e Expression of MeAHL17 in cassava leaves transformed with pCAMBIA1304 (vector control, VC1), pCAMBIA1304::MeAHL17 (OE), pTRV (vector control, VC2), or pTRV::MeAHL17 (RNAi) in four cultivars, respectively, as detected by qRT-PCR (*P < 0.05, **P < 0.01; two-tailed t-test). Data are represented as mean ± s.d. (n = 3 biological replicates). f The bacterial number in cassava leaves transformed with pCAMBIA1304 (vector control, VC1), pCAMBIA1304::MeAHL17 (OE), pTRV (vector control, VC2), or pTRV::MeAHL17 (RNAi) in four cultivars at 0 and 6 days post inoculation (*P < 0.05, **P < 0.01; two-tailed t-test). Data are represented as mean ± s.d. (n = 4 biological replicates)