Fig. 3

Single-cell domains persist under perturbations of epigenetic components and transcription machinery (target region as stated in Fig. 1). A Single-cell domains with HDAC inhibitor TSA + NaBu treatment (leading to hyper acetylation of histones). B Single-cell domains with DMOG treatment (leading to hyper methylation of histones). C Single-cell domains with GSK126 treatment (leading to lower H3K27me3). D Single-cell domains with α-amanitin treatment (leading to inhibition of transcription). E Single-cell domains with ZdCyd treatment (leading to inhibition of methylation of cytosine-C5 of DNA). Gray rows and columns in A–E indicate undetected loci. The yellow lines in A–E represent the ensemble TAD boundary. F Average boundary frequencies with or without TSA + NaBu, DMOG, GSK126, α-amanitin, and ZdCyd treatments. Error bars stand for standard deviation. G Boundary strengths with or without TSA + NaBu, DMOG, GSK126, α-amanitin, and ZdCyd treatments. For each box, the horizontal lines from top to bottom represent the non-outlier maximum, 75% quantile, median, 25% quantile, and non-outlier minimum. Outliers are defined as values that are more than 1.5 times the interquartile range away from the bottom or top of the box. In F and G from left to right: N = 483, 477, 182, 201, 248, 319, 186, 183, 163, 188, 111, 115. H A summary of all adjusted p values calculated in F and G. All p values were calculated using two-sided Student’s t tests with false discovery rate correction