Fig. 5

A high-resolution segregation analysis revealed the absence of crossing over in Sd. ludwigii meiosis. a Experimental setup (further details in Additional file 2: Fig. S4a). b Genomic comparison of the haploid parental strains. c Results of the genome-wide SNP segregation analysis for a representative tetrad. Each dimorphic position of the genome was assigned to one of the parental haplotypes (red for the reference strain, blue for spore 122). Genomic regions with high and low levels of sequence divergence are indicated with solid and dashed lines (underneath), respectively. d Two verified single-marker NCOs were detected by the SNP segregation analysis. e A single LOH event was detected in tetrad 985–988, in close proximity to another shorter region with unusually low SNP density. f Immunostaining of meiotic nuclear spreads of a Sd. ludwigii strain expressing Rap1-GFP using anti-Rad51 and anti-GFP antibodies. Bar: 1 μm