Fig. 2

Genome structure of Sd. ludwigii and mapping of previously used genetic markers. a The nuclear genome of the haploid reference strain NBRC 1722 is organized in 7 chromosomes, resolved as chromosomal bands on a PFGE gel (left) and depicted as bars (right). The only gap in the assembly (in chrB) corresponds to the internal part of the rDNA region, which is flanked by assembled rDNA repeats. b Genomic comparison of the Sd. ludwigii parental strains used in the previous genetic analyses. c The 7 previously defined Sd. ludwigii linkage groups [12] were matched to the 7 chromosomes of the reference genome assembly, and 16 of the previously used markers were unambiguously mapped on the assembly (yellow circles). The MAT locus is shown as a blue circle (in chrE). One of the rare COs that were previously detected using tetrad analysis was tracked down to a reciprocal exchange event between the marker “ade3” (corresponding to the ADE4 gene) and the centromere of chrF, depicted here as an example