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Fig. 2 | Genome Biology

Fig. 2

From: Lamin C is required to establish genome organization after mitosis

Fig. 2

Specific knockdown of lamin C causes LASes to delocalize from the nuclear periphery and can be rescued by re-expression of lamin C. A Representative images showing the disposition of EGFP-LacI bound lacO arrays (arrowheads, green) and lamin B1 (red; lamin A/C for shB) in the TCIS clones Y (top) and 12 (bottom) pre- and post-integration of the Ikzf LASI, which drives lamina-association, and indicated lamin isotype depletion. The inset shows 300× magnification. B Quantitation of peripheral association was determined by overlap of EGFP-LacI bound foci and lamin B1 (or lamin A/C for shB1; n ≥ 50). Error bars indicate SD and statistically significant differences in peripheral association of treatment conditions when compared to shCtrl (p ≤ 0.001) are indicated by asterisks. C Quantitation of peripheral association in rescue experiments was determined as in B. D Representative images showing the disposition of lacO arrays in rescue experiments as in A

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