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Fig. 4 | Genome Biology

Fig. 4

From: IRFinder-S: a comprehensive suite to discover and explore intron retention

Fig. 4

Differential Intron retention validation between stages of EMT differentiation. A EMT is induced in MCF10a Snail-ER system. RNA-seq data is analyzed with IRFinder, and the replicates are compared using DESeq2 and SUPPA2 wrappers. B qPCR validation of 12 introns called as differentially retained by DESeq2 and/or SUPPA2. The complete list of primers and characteristics of each intron is described in supplementary file 1. C Scatter plot showing the IRratios of the introns called as differentially retained by DESeq2, in green, SUPPA2, in orange, and by both in yellow

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