Fig. 2

Validation of the top 10 hits from surfaceome and genome-wide screens. a Cell viability assay for determination of the protective effects of identified gene knockout on RV-B14-induced cell death. The assay is performed at 24 h post infection of RV-B14 at an MOI of 2. b RT-qPCR quantification of viral loads in medium supernatant. The supernatant is harvested at 24 h post infection of RV-B14 at an MOI of 2. c Immunofluorescence (IF) staining of RV-B14 envelope protein for evaluation of infection rates at individual cell lines. IF staining is performed at 16 h post infection of RV-B14 at an MOI of 2. Each biological replicate contains the quantification results from 2000 cells. d, e RT-qPCR quantification of viral loads in medium supernatant (d) and the lysate (e) of mock and knockout single clones. The supernatant and cell lysate are harvested at 24 h post infection of RV-B14 at an MOI of 2. The significant difference between knockout cells and non-targeting sgRNA mock groups are determined using two-tailed unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001