Fig. 4

Transcriptomic effects of QKI5 protein overexpression in THP-1 cells. a Schematic diagram of experimental setup prior to RNA sequencing. b Scatterplots showing TPM values of differentially expressed genes (DEGs) between QKI5-overexpressing and Ctrl THP-1 cells (left panel), QKI5 M1-overexpressing and Ctrl THP-1 cells (middle panel), and PMA-treated (PMA 48 hr) and Ctrl THP-1 cells (right panel). c Scatterplots showing log2 (fold change) of up- and downregulated DEGs after QKI5 and QKI5 M1 overexpression. The correlation of gene differential expression between QKI5 regulated and QKI5 M1 regulated genes was calculated by Pearson correlation coefficient. d Point-by-point comparison of DEGs in QKI5- and QKI5 M1-overexpressing THP-1 cells and PMA-treated THP-1 cells. The correlations of gene differential expression between QKI5, QKI5 M1 regulated genes, and DEGs upon PMA treatment were calculated by Pearson correlation coefficient. e Venn diagram showing the comparison of DEGs (upper panel: commonly activated; lower panel: commonly repressed) among, QKI5-overexpressing vs. Ctrl (QKI5-activated/-repressed), QKI5 M1-overexpressing vs. Ctrl (QKI5 M1-activated/-repressed) and PMA vs. Ctrl (PMA-activated/-repressed). Heatmap on the right represents the scaled log2 (fold-change) of differential expression of commonly activated and commonly repressed genes. f, g GO functional enrichment analysis of commonly activated (f) and commonly repressed genes (g)