Fig. 3

Short-read Split-seq analysis. a UMAP of 36,869 short-read Split-seq cells/nuclei labeled by 20 Leiden clusters (R) with RNA velocity field trajectories and breakdown of cell type per cluster with number of cells per cluster: 7797 0 h myoblast cells (pink), 10,194 0 h myoblast nuclei (blue), 18,878 72 h nuclei (Pax7^hi in green and Myog^hi in dark green). b UMAP of short-read Split-seq cells/nuclei with the 464 cells with matching long reads in color corresponding to R1-R20. c Histogram of the number of the 464 cells/nuclei per R1-R20. d Distribution of expression of marker genes; dark blue = lowly expressed, yellow = highly expressed. e Visualization of transcripts in mononucleated cells and myotubes at the 72 h differentiation timepoint. Blue = DAPI, pink = Myh3, green = Col1a1, yellow = Itm2a. Scale bar: 50 μm