Fig. 7
RNA-seq analysis following oncogenic KrasG12D induction. a Venn diagram of differentially expressed genes (DEGs) at 12, 24, and 48 hrs. DEGs were classified based on expression fold change ≥ |2| and FDR ≤ 0.05. Total number of DEGs across time points: 2091. b RPKM expression levels of DEGs normalized to the Z scale and plotted for 0, 12, 24, and 48 hrs. Yellow: positive change. Blue: negative change. Black: no change. c Pathway enrichment analysis of DEGs for 12, 24, and 48 hrs using RITAN and MSigDB hallmark gene set collection. Color scale − log10(FDR). Gene expression networks obtained from upstream regulatory analysis at 24 hrs in Ingenuity Pathway Analysis (IPA) software for downregulated d TGFB1, and upregulated e MYC and f KRAS. The blue and green gene shapes and lines indicate predicted or observed inhibition respectively, while the yellow line indicates the predicted relationship is inconsistent with gene expression. The orange and red gene shapes and lines indicate predicted activation or observed upregulation respectively. Gray lines indicate no predicted effect