Fig. 2
Analysis of cancer signaling phospho-antibody array containing 269 antibodies following oncogenic KrasG12D induction. a Venn diagram of differentially phosphorylated proteins (DPPs) at 12, 24, and 48 hrs with signal fold change ≥ |1.5|. b Signal ratios of DPPs normalized to the Z scale and plotted for 0, 12, 24, and 48 hrs. c Pathway enrichment analysis of DPPs for 12, 24, and 48 hrs using the Molecular Signatures Database (MSigDB) hallmark gene set collection. Color scale represents standardized –log10(FDR) values. d Protein-Protein interaction network obtained from DPP using the STRING database and interaction confidence score ≥ 999. Center blue circle: downregulated protein phosphorylation log2(fold change). Center yellow circle: upregulated protein phosphorylation log2(fold change). Some proteins have multiple phosphorylation sites and, hence, will have multiple colors in the center circle. Outer concentric circles represent activating (orange) or inhibiting (light blue) protein activity. The effect on activity by the combination of the mark’s effect and its level were interpreted. For instance, an inhibitory mark decreasing should lead to a more active protein. Some proteins have both inhibitory and activating phosphorylations and, hence, will have multiple colors in the outer concentric circle. Protein names highlighted in bold are mentioned in the text with their fold changes