Fig. 4

SRCP enables accurate identification of more differentially expressed circRNAs than other pipelines. A Relative amount of total circRNAs reads (for true circRNAs) in young (1 day old) and aged (20 days old) flies. The total number of circRNAs was calculated using the individual pipelines, and reads assigned to true circRNAs were added up for each condition. The average of young flies was normalized to 1. * Indicates significance (t-test, p value < 0.05), while NS indicates no statistically significant differences. The error bars represent the standard error of the mean (SEM). B Number of true (violet bars) and false-positive (orange bars) differentially expressed circRNA found by SRCP and the other circRNA-identification pipelines. C Validation of DE circRNAs by qPCR. Expression of target circRNAs and beta-Tubulin mRNA were normalized on the level of TBP mRNA. We then plotted the average of 3 independent biological replicates and the error bar represents the SEM. The average of young flies (1 day old) was normalized to 1. We performed t-test to compare 1 day old vs 20 days old. * Indicates p value < 0.05, ** p value < 0.005, NS non-significant