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Fig. 6 | Genome Biology

Fig. 6

From: Differential regulation of mRNA fate by the human Ccr4-Not complex is driven by coding sequence composition and mRNA localization

Fig. 6

Ribosome pause sites regulated by the Ccr4-Not complex. A Venn diagram indicates the number of mRNAs that contain pause sites sustained, induced, or resolved following the depletion of CNOT1. B–D Examples of individual mRNAs with different Ccr4-Not regulated ribosome pause types. B Pause resolved in the absence of CNOT1. C Paused sustained in the presence and absence of CNOT1. D Paused induced by CNOT1 knockdown. Plots show RPF coverage normalized for mRNA abundance (TPM). E The change in mRNA half-life after CNOT1 knockdown for the groups of mRNAs with different pause types identified in (A). F mRNAs with resolved pause sites in the absence of CNOT1 shown an increase in protein production relative to the ribosome occupancy on the mRNA when CNOT1 is depleted. G–I Amino acid sequence motifs at the E, P, A sites of paused ribosomes and three codons upstream and downstream generated using Seq2Logo [111], shown for resolved pauses (G), sustained pauses (H), and induced paused (I)

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