Fig. 5

Nuclear proteins enriched for disorder-promoting AAs are translationally upregulated. A Heatmap represents the synonymous codon usage of each codon that encodes a given amino acid and a column z-score has been applied to compare the codon preference between the groups of mRNAs. The colored bar at the top indicates the nucleotide present at the third position in the codon. B Heatmap shows the average frequency of each amino acid per mRNA and a column z-score has been applied. The colored bar at the top classifies the amino acids by their side chain type. C The percentage of amino acids encoded by the mRNAs that promote disorder in protein structure [106, 107]. Key significant comparisons are shown, and all statistical comparisons are presented in Additional File 9: Fig. S2A. D The localization of disorder-promoting amino acids across the CDS for the groups of mRNAs with minimal changes in mRNA stability. E Protein class enrichment analysis (using PANTHER [110]) shows that the mRNAs with increased TE but minimal increase in stability are enriched for transcription factors, specifically C2H2 zinc-finger proteins