Fig. 6

Dissection of the constituent elements within TH SEs. a An illustration of the early, intermediate, and late elements within TH SEs. b Heatmap showing the differential motif enrichment pattern for the three types of elements within TH SEs as well as the elements in Con and DN SEs. Color represents the –log10 (P value). c Genome browser snapshot showing the progressing landscape of the Dnajb12 SE locus during erythroid cell differentiation. Early and late enhancers are defined according to the temporal order of emergence. d ATAC-seq data showing chromatin accessibility landscape of the SE locus in EML and MEL cells. EML and MEL cells represent the hematopoietic pluripotent cells and differentiated erythroid cells, respectively. The early enhancer is activated in EML and MEL cells, while the late enhancer is activated in MEL cells. e Comparison of gene expressions between wild type (WT) and enhancer-knockout EML cells. **P < 0.01; n.s., not significant. . P-values are determined by using the Student’s t test. f Comparison of gene expressions between wild type (WT) and enhancer-knockout MEL cells. **, P < 0.01; n.s., not significant.  P-values are determined by using the Student’s t test