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Fig. 4 | Genome Biology

Fig. 4

From: H2A ubiquitination is essential for Polycomb Repressive Complex 1-mediated gene regulation in Marchantia polymorpha

Fig. 4

H2Aub contributes to PRC1-mediated transcriptional expression. a Venn diagram showing overlap of upregulated genes in Mph2a;H2AK115R/K116R, Mph2a;H2AK119R, and Mpbmi1/1l mutants. Significance was tested using a Hypergeometric test. b Venn diagram showing overlap of downregulated genes in Mph2a;H2AK115R/K116R, Mph2a;H2AK119R, and Mpbmi1/1l mutants. c Boxplot showing expression level (RPKM, reads per kilobase per million mapped reads) of genes in overlap 1 (panel a) in WT, Mph2a;H2AK115R/K116R, and Mpbmi1/1l. d Boxplot showing expression level (RPKM) of genes in overlap 2 (panel a) in WT, Mph2a;H2AK119R, and Mpbmi1/1l. e Boxplot showing expression level (RPKM) of genes in overlap 3 (panel b) in WT, Mph2a;H2AK115R/K116R, and Mpbmi1/1l. f Boxplot showing expression level (RPKM) of genes in overlap 4 (panel b) in WT, Mph2a;H2AK119R and Mpbmi1/1l. g–j Boxplots showing H2Aub levels of genes in the group of overlap 1 (g), overlap 2 (h), overlap 3 (i), and overlap 4 (j). H2Aub levels were calculated as the average RPKM from 1 kb upstream of the transcriptional start to the transcriptional start of genes. k–n Boxplots showing H3K27me3 levels of genes in the group of overlap 1 (k), overlap 2 (l), overlap 3 (m), and overlap 4 (n). H3K27me3 levels were calculated as the average RPKM from 1 kb upstream of the transcriptional start to the transcriptional start of genes. Boxes show medians and the interquartile range, and error bars show the full range excluding outliers. n.s., not significant; *p < 0.05, **p < 0.01 (Wilcoxon test)

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