Fig. 5

Functional validation of the role of CTCF on repressing BLCAP expression. A Cas9-expressing SEM cells infected with sgRNA against CBS were collected for genomic PCR and Sanger sequencing. Indel frequency was evaluated by Inference of CRISPR Edits (ICE). B Q-PCR was conducted in sgBLCAP-CBS targeted bulk population and sgRNA-NT control using primers against BLCAP mRNA. C Quantification of mRNA change of BLCAP upon CTCF loss by RNA-seq. D Q-PCR validation was conducted to examine the mRNA expression of BLCAP in response to acute depletion of CTCF protein by auxin treatment for 24 and 48 h and washout. *p value < 0.05; **p value < 0.01; ****p value < 0.0001. Student’s t test