Fig. 4
From: Acute depletion of CTCF rewires genome-wide chromatin accessibility
Integrated analysis of putative insulator CTCF-binding sites. A Scheme of integrative analysis to identify 67 putative CTCF insulators at target genes. B Screenshot of chromatin status characterization of the BLCAP gene and adjacent chromatin regions. Publicly available ChIP-seq tracks were included to annotate the transcription factor occupancy at this region. Blue and orange bars depict the annotated left and right chromatin looping anchors identified by Hi-C. C Screenshot of the BLCAP gene and surrounding chromatin regions with ATAC-seq tracks of CTCFAID cells with and without IAA treatment. Averaged tracks are combined analyses of three individual clones—RNA-seq tracks of CTCFAID cells with and without IAA treatment. Publicly available CTCF ChIP-seq tracks were included to indicate CTCF occupancy in this region. WGBS tracks from clone 27 serve as a negative control because no methylation occurred in this region