Skip to main content
Fig. 6 | Genome Biology

Fig. 6

From: Time-dependent effect of 1,6-hexanediol on biomolecular condensates and 3D chromatin organization

Fig. 6

Short-term 1,6-HD treatment respectively enlarged and shortened the TADs in A and B compartments. A, B Left: average size of TADs in compartment A (A) or compartment B (B). Right: alluvial plot of TAD reorganization in compartment A (A) or compartment B (B) during 1,6-HD treatment. Line thickness is proportional to the number of TADs with a specific type of reorganization compared to 0 min. All FDR values were determined by diffHic (details in “Materials and methods”). C, D Density of CTCF ChIP-seq peaks in unchanged, lost, or gained boundaries in compartment A (C) or compartment B (D) across all time points compared to 0 min. E, F Difference of aggregate TAD analysis (ATA) signal versus 0 min. TADs in compartment A (E) or compartment B (F) are resized to a uniform size and calculated the average interactions. G, H Examples (G: chr2:167–169 Mb, H: chr8: 98.44–106.56 Mb) showing TADs in compartment A (G) or compartment B (H) at 0 min, 2 min, 30 min, and withdrawal. Bottom: CTCF ChIP-seq signal and PC1. I Schematic indicating that short-term 1,6-HD respectively enlarged and shortened the TADs in A and B compartments

Back to article page