Fig. 3

Short-term 1,6-HD treatment strengthened compartmentalization. A Average contacts between 100 kb bins from the same (A-A, B-B) and different (A-B) compartment types at each time point. The semi-transparent yellow shadow highlights the results at 2 min. B Compartment strength of all 100 kb bins from A-, B-, and both compartment types at each time. The semi-transparent yellow shadow highlights the results at 2 min. C Example (chr14: 45–80 Mb) showing compartmentalization strengthened at 2 min and weakened at 30 min. Top: eigenvector (PC1), red part refers to A compartment and blue part refers to B compartment. Middle: Pearson’s correlation maps in the upper right and observed contact matrices in the bottom left. Bottom: per-bin compartment strength, per-bin average contacts between loci with same type (A-A or B-B), per-bin average contacts between loci with different types (A-B). D The proportion of strengthened (with at least 2-fold enhanced compartment strength) and weakened (with at least 2-fold reduction in compartment strength) bins compared with 0 min. E Schematic of the short-term 1,6-HD effects on compartmentalization