Fig. 2

Short-term 1,6-HD treatment decreased long-range interactions and increased short-range interactions. A Contact probability in logarithmic genomic distance. The two zoom-in boxes show greater details around 0.1 Mb and 10 Mb. B Fold change of relative contact probability (RCP) versus 0 min at the distanced annotated on the upper left of each plot. Error bars represent SD from 20 chromosomes (chr1-chr19, chrX). All p values were determined using paired Student’s t test. C Quantification of changed percentage of interactions at indicated genomic range in the biological replicates compared with 0 min (details in “Materials and methods”). D The leftmost column: observed contact matrices for chr8 in untreated cells. Other columns: differential contact matrices for each time point minus 0 min. The bold black line box highlights example regions at distance of 100 Mb, 10 Mb, 1 Mb, and 100 kb respectively