Fig. 2

Variable chromatin accessibility across founder DO strains. a Experimental overview and schematic of primary output for chromatin accessibility profiling of founder DO strains by ATAC-seq and differential accessibility analysis. b, c Genome browser displays of differentially accessible ATAC-seq peaks. b A differentially accessible distal intergenic ATAC-seq peak (translucent gray) and a CAST-PWK specific ATAC-seq peak at the Adcy5 intron (translucent blue). c A differentially accessible ATAC-seq peak at the Nomo1 promoter (translucent red) and an ATAC-seq peak less accessible in PWK at the Abcc8 intron (translucent gray). d Heatmap of Pearson correlations between each pair of samples based on normalized chromatin accessibility cluster strains consistent with their genetic relatedness. Hierarchical clustering reveals the two clusters of strains outlined in black. e Differentially accessible regions (rows) in 16 samples (columns) of eight founder DO strains across two sexes. ATAC-seq peak scores are standardized to the [0, 1] range. Rows are clustered by k-means (k = 10). The six wild-derived clusters from top to bottom are: PWK, CAST-PWK-WSB, CAST-WSB, and CAST, absent in CAST-PWK, WSB. Additional file 1: Figure S2 is the full version of this figure