Fig. 3

Genome wide mapping of the Platr10 target gene network by RAT-seq. A Schematic diagram of the RNA reverse transcription-associated trap sequencing (RAT-seq) assay. Platr10 lncRNA was labeled with biotin-dCTP in situ reverse transcription using three Platr10-specific complementary primers. The biotin-Platr10 chromatin complex was isolated by streptavidin beads and the Platr10-binding target DNAs were isolated for Illumina library sequencing. This RAT-seq generates a Platr10 genome wide target DNA network. B Platr10 target gene interacting network. The Platr10 target pathway genes were mapped by gene ontology analysis. Platr10 binds to all four stem cell-associated factors that have been used to reprogram somatic cells: Oct4, Sox2, Klf4, and c-Myc