Fig. 3

ExOrthist output and benchmarking. a Statistics of the exon orthogroups (OG) from an ExOrthist main run on three mammalian species (human, mouse, cow) as generated by get_cluster_stats.pl. b Percentages of conserved human coding exons based on their location within the gene (first, internal, last) or on their average inclusion level (for internal exons only), as obtained from VastDB [29]. c Venn diagrams showing the number of exons (black) included in either ExOrthist orthogroups (green area), liftOver-based orthogroups (pink area) or both (intersection) for human, mouse, and cow. The percentages of shared and not-shared exons between ExOrthist and liftOver-based orthogroups are reported in the corresponding area. The percentage in brackets refers to the concordance between the 1:1 exon pairs in the target species (e.g., mouse and cow for human exons) retrieved by both methods. d Plot of the exon-intron structure of human MIS18A and its mouse and cow orthologs as generated by the exint_plotter module. Exons in the different species falling in the same exon clusters are vertically aligned, and a randomly chosen exon of interest is highlighted in green. Intron positions are depicted as colored dots, depending on the phase (0: red, 1: blue, 2: green). First and last exons are shown as arrows, and the length of the CDS portion of each human MIS18A exon (in nucleotides) is reported on top