Fig. 1

Overview of simulation study and comparison of four similarity metrics. A One BED file was used to create 360 perturbed files, 10 repetitions for each of 36 different combinations of add, drop, and shift perturbations. B Examples of a demo region set that has been shifted to different degrees. C We calculated four similarity metrics between the original file and the perturbed files. The greater the similarity score decrease, the more sensitive the metric is. D Within each parameter group, as the perturbation increases, the similarity score decreases. E Results for shift, drop, and add-only perturbations. L, low; M, moderate; H, high perturbation