Fig. 4
From: Nanopore sequencing reveals endogenous NMD-targeted isoforms in human cells
(A) Cumulative distribution function (CDF) of the fraction of NMD-sensitive (red) and NMD-insensitive transcript isoforms (blue) with specified numbers of exons in the 3΄UTR. The statistical significance of the difference between the two distributions was determined by the K-S test, p=7x10-202, N=1'911 (B) CDF of the fraction of NMD-sensitive and NMD-insensitive transcript isoforms with specified 3´UTR length. Statistical significance was determined by K-S test, p=4x10-91, N=1'911 (C) CDF of the fraction of NMD-sensitive and NMD-insensitive transcript isoforms with a specified length of the 3΄UTR, restricted to isoforms with an APPRIS-designated termination codon in the last exon. Statistical significance was determined by K-S test, p=0.36, N=389 for NMD sensitive and N=1'311 for NMD-insensitive transcripts. (D) Relative percentage of experimentally validated uORFs in NMD-sensitive and NMD-insensitive mRNA isoforms computed separately for all isoforms (blue) and for those with a termination codon in the last exon (red). The numbers of transcripts in the different categories are indicated on the plot