Fig. 5
From: MAAPER: model-based analysis of alternative polyadenylation using 3′ end-linked reads
Single-cell analysis by MAAPER using a paired test. A Number of genes with significant 3′UTR shortening in EVTs vs. VCTs (left) or SCTs vs. VCTs (right) for the number of donors showing significant regulation. B As in A except that number of genes with significant 3′UTR lengthening is shown. C Median REDu scores calculated for SCTs vs. VCTs and EVTs vs. VCTs (Vento-Tormo et al. data) across five individuals. Only genes that showed significant APA changes in corresponding samples are included for plotting. Error bars indicate the SD of median values across donors, and P values are based on the one-sided t tests. D SD of REDu scores using significant genes identified by paired or unpaired tests for EVTs vs. VCTs (left) or SCTs vs. VCTs (right). FDR-adjusted P value = 0.1 is used as a threshold to select significant genes. Displayed P values are based on the Wilcoxon rank sum tests. E, F P-value distributions in paired or unpaired tests for EVTs vs. VCTs (E) or SCTs vs. VCTs (F). G, H Example genes MUC15 (G) and CEP120 (H). Aggregated reads from two donors (D8 and D11) are displayed. P values of RED scores are based on the one-sided t tests