Fig. 4

Spatial distributions of CTCF, MCM2, and PCNA relative to the early replicating TADs in the G1 and G1/S phases. a–c Representative STORM images of CTCF, MCM2, and PCNA labeled by immunostaining (purple) and metabolically labeled TADs (green). Cells were fixed and labeled in the mid-G1 phase (upper) or G1/S phase (lower). The areas inside the yellow squares are shown at higher magnification next to each nucleus. Portions of the two signals that overlap are shown in white. d Barycenter distances between CTCF, MCM2, or PCNA with the TADs in the mid-G1 phase or G1/S phase. e Radii of CTCF, MCM2, or PCNA foci in the mid-G1 phase or G1/S phase. For lines and statistics in d and e, see the description in the legend of Fig. 1 (n = 10 cells)