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Fig. 4 | Genome Biology

Fig. 4

From: Coordinated changes in gene expression kinetics underlie both mouse and human erythroid maturation

Fig. 4

In vivo analysis of Gata1 function using a chimaera assay coupled with scRNA-Seq. A Schematic of the G1ER system [29, 30]. B Behavior of the 89 MURK genes identified in Fig. 3 upon Gata1 induction in the G1ER system [28]. Wu et al. report that upon Gata1 induction they obtained a total of 2769 upregulated genes, 6079 mildly upregulated, 3566 downregulated, and 3445 with no response. C UMAPS of Gata1 chimera cells allocated a hemato-endothelial identity colored by cell-type (sub-clusters defined in Pijuan-Sala et al. [25]—BP: blood progenitors, EC: endothelial cells, Haem: hemato-endothelial progenitors, Mk: megakaryocytes, My: myeloid cells, Ery: erythroid cells) and split by genotype. Orange arrowheads highlight increased population with megakaryocytic signature in Gata1 fraction. D UMAPS of Gata1 chimera cells allocated a hemato-endothelial identity colored by sampling timepoint and split by genotype. E Barplots with the quantification of chimera cells mapping to each hemato-endothelial lineage of the reference dataset (left) and to sampled timepoints of the reference dataset (right)

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