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Fig. 2 | Genome Biology

Fig. 2

From: Direct long-read RNA sequencing identifies a subset of questionable exitrons likely arising from reverse transcription artifacts

Fig. 2

The detection of questionable exitrons is common in cDNA-seq and dcDNA-seq. a Schematic representation of the workflow to identify falsitrons in public ONT sequencing datasets. b Genome browser view showing the falsitron in TAX1BP3 in ONT sequencing data for GM12878. c Violin plots indicating the detection of falsitrons in cDNA-seq and dcDNA-seq of different human cell lines. d Stacked bar plots showing the fraction of falsitrons of different lengths. e Bar graph depicting the length of falsitron-flanking direct repeats. f Violin plots show relative abundance of falsitron products in DNAJC22 and GAS2L3 for three TCGA cancer cohorts. ESCA, esophageal carcinoma. OV, ovarian serous cystadenocarcinoma. STAD, stomach adenocarcinoma. g Plot showing cumulative percentage with direct repeats of at least a given length. Dashed lines indicate the total fraction of introns with direct repeats (≥ 4 nt). h Sequence logos indicating nucleotide composition at 5′ and 3′ splice sites. Positions of splice site dinucleotide motifs are highlighted

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Genome Biology

ISSN: 1474-760X

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