Fig. 1
From: The dynamics of N6-methyladenine RNA modification in interactions between rice and plant viruses
Flow chart of the investigation of m6A methylation during infection of rice by RBSDV or RSV. A Symptoms of RBSDV- and RSV-infected rice plants in plastic buckets at 60 days post infection (dpt). The left plant is a mock-treated plant, the middle two plants are infected with RBSDV, and the plant on the right is infected with RSV. B RT-PCR and western blot (WB) detection of RSV using a specific pair of primers corresponding to RdRp and anti-NS3 specific antiserum. Total proteins were stained with Coomassie brilliant blue (CBB), which was treated as the loading control. C RT-PCR and WB were performed to detect RBSDV in rice using a specific pair of primers corresponding to CP. Anti-p10 specific antiserum was carried out for the WB. D Experimental flow chart of m6A-IP-seq and RNA-seq using RBSDV- and RSV-infected plants. NGS, next-generation sequencing. MeRIP, methylated RNA immunoprecipitation