Fig. 5

Bacterial and fungal isolates from the Challenger Deep sediment habitat. A Phylogeny and percentage of bacterial and fungal isolates (Additional file 1: Table S12 and S13). B Visualization of representative bacterial isolates from four different phyla. Cells were cultured under 0.1 and 100 MPa, respectively. Scale bars, 10 μM; DIC, images taken with a DIC microscope; DAPI, cells stained with DAPI and observed with an epifluorescence microscope. C Visualization of representative fungal isolates from nine genera. Scale bars, 30 μM; CW, cells stained with Calcofluor White and observed with an epifluorescence microscope. The phylogeny of fungal isolates is shown to the right. Red arrows indicate swollen hypha (MT19_19) or swollen conidia (MT20_3)