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Fig. 5 | Genome Biology

Fig. 5

From: Positive natural selection of N6-methyladenosine on the RNAs of processed pseudogenes

Fig. 5

Experimental validation of m6A on processed pseudogene DSTNP2 affects the expression of DSTN in GM12878 cell line. a Relative gene expression of DSTNP2 and DSTN after DSTNP2 knockdown using siRNA. * P< 0.05; *** P< 0.001 (two-tailed t test). b Western blot comparing the protein levels of DSTN in control and DSTNP2 knockdown cells. c The relative expression of DSTN upon overexpression empty vector and wild type DSTNP2 respectively. Error bars represent standard errors. *** P< 0.001 (two-tailed t test). d The relative expression of DSTN at different time points after transcription inhibition in control and DSTNP2 knockdown cells respectively. Error bars represent standard errors. * P< 0.05; ** P< 0.01; *** P< 0.001 (two-tailed t test). e The relative expression of DSTN and DSTNP2 after adding control inhibitor versus miR362-5p inhibitor. f Bar plot comparing the relative expression of total, cytoplasmic and nuclear DSTN respectively upon overexpression of wild type versus m6A sites-mutated DSTNP2. g Western blot comparing the protein levels of DSTN upon overexpression of wild type DSTNP2 versus m6A site-mutated DSTNP2. h, i The relative expression of DSTNP2 (h) and DSTN (i) upon overexpression of wild type DSTNP2 versus m6A site-mutated DSTNP2 at different time points after transcription inhibition. Error bars represent standard errors. ** P < 0.01; *** P < 0.001 (two-tailed t test). j The relative expression of DSTN upon overexpression of wild-type DSTNP2 versus m6A site-mutated DSTNP2 after adding control inhibitor and miR362-5p inhibitor respectively. Error bars represent standard errors. * P < 0.05 (two-tailed t test). k The relative expression of DSTN at different time points after transcription inhibition upon overexpression of wild-type DSTNP2 versus m6A site-mutated DSTNP2 after adding control inhibitor and miR362-5p inhibitor respectively. Error bars represent standard errors. ** P< 0.01; *** P< 0.001. l Western blot showing the protein level of DSTN upon overexpression of wild type DSTNP2 versus m6A site-mutated DSTNP2 after adding control inhibitor and miR362-5p inhibitor, respectively

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