Fig. 1

Identification of translational efficiency QTLs in the HXB/BXH panel. A Schematic overview of the establishment of the HXB/BXH recombinant inbred panel. Colored bars represent SHR and BN-Lx alleles. B Schematic overview of the experimental procedures carried out for each of the 30 HXB/BXH lines. C Bar plot illustrating absolute and relative ORF identifications, separated by coding and noncoding gene biotypes, for heart and liver. Translated pseudogenes were excluded from downstream analyses. See also Additional file 2: Table S1. D Table with gene-centric QTL mapping results for heart and liver, separated by genes for which mRNA expression QTLs (eQTLs), ribosome occupancy QTLs (riboQTLs), and/or translational efficiency QTLs (teQTLs) are identified. Local QTLs indicate associations that map to the same genomic locus as the tested gene (see “Methods”). Distant QTLs refer to associations with genes on chromosomes other than that of the QTL. See also Additional file 3: Table S2. E Venn diagrams displaying a gene-centric overlap of eQTLs, riboQTLs, and teQTLs in heart and liver, highlighting QTLs shared with, or specific to, a single trait. F Bar plot with a tissue comparison of detected local translational efficiency QTLs (teQTLs) considering only genes expressed and translated in both tissues. Genes are ordered by the delta p value in heart vs. liver tissue (middle panel). Three examples of genes expressed in heart and liver tissue are given, displaying a local teQTL in either one, or both, of these tissues. Cross bars indicate mean values. See also Additional file 1: Figures S1-S2 and Additional files 2, 3, 4: Tables S1, S2, S3