Fig. 3

NUMT on A188 nuclear genomes. a NUMT sequence on 10 chromosomes of A188Ref1. Each dot on chromosomes designates a potential NUMT integration. Close-up alignments with the mitochondrion (mt) genome are shown along NUMTs. Each alignment requires at least 5 kb match and 95% identity. b Circos plot of alignments between the mt genome and 10 chromosomes. The same colors of green, orange, dark blue label duplicated regions in mt. “P” regions match the probe sequence used for FISH. Brown links highlight alignments on chromosomes 8 and 10. Note that the chromosomal scale is different from the mt scale. Numbers on the track are in Mb. c Physical mapping of a mt DNA (mtDNA) and knob repeats on the mitotic metaphase chromosomes of maize A188. The knob repeat probe (green signals) was used to identify the chromosomes. Two FISH sites of the mtDNA insertion on the chromosomes were detected: arrowheads, chromosome 8; arrows, chromosome 10. Bar = 10 μm