Fig. 3

Example of the pooled amplification paradox as seen in an analysis of the EVAL dataset. A Histograms of the counts per UMI for two genes with different amplification. The histograms are generated from downsampled data (4 times), visualized by a dashed line in B and C. B Change in the mean gene expression estimates across cells of the two genes when downsampling the reads. C Change in gene expression for downsampled data when correcting for unseen molecules. The code to reproduce this figure is here: https://github.com/pachterlab/GRNP_2020/blob/master/notebooks/figure_generation/GenFig1_3.ipynb