Fig. 7

Influence of m⁶A on translation initiation factors or elongation factors in strawberry. a Integrated Genome Browser (IGB) tracks displaying the distribution of m⁶A reads in transcripts of genes encoding translation initiation factors (EIF2, EIF2B, EIF3A, and EIF3C) and elongation factors (EF1A). The hypermethylated m⁶A peaks (fold change ≥ 1.5; P value < 0.05) in fruit at RS1 stage compared to those at S6 stage are indicated by shadow boxes. S6, the growth stage 6; RS1, the ripening stage 1. Rep, replicate. b m⁶A enrichment for EIF2, EIF2B, EIF3A, EIF3C, and EF1A from m⁶A-seq data. c Transcript levels of EIF2, EIF2B, EIF3A, EIF3C, and EF1A determined by RNA-seq. FPKM, fragments per kilobase of exon per million mapped fragments. d RNA immunoprecipitation (RIP) assay revealing the binding of MTA protein to the transcripts of EIF2, EIF2B, EIF3A, EIF3C, and EF1A. e–i The changes in relative m⁶A enrichment (e, g), gene expression (f, h), and mRNA stability (i), for EIF2, EIF2B, EIF3A, EIF3C, and EF1A in the MTA-silenced (RNAi-MTA) or MTA-overexpressed (OE-MTA) fruits. The relative m⁶A enrichment and gene expression were determined by m⁶A-IP-qPCR and quantitative RT-PCR, respectively. The ACTIN gene served as an internal control. For mRNA stability assay, the total RNAs were extracted after actinomycin D treatment at an indicated time point and subjected to quantitative RT-PCR assay. Data are presented as mean ± standard deviation (n = 3). Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t test)