Fig. 4

Comparison of mutation frequencies in various genomic regions among the Control, Heat, and Warming lines (a) and populations (b). The numerical values in the stacked bar chart indicate the frequencies of total mutations (SNVs and indels) in the genomic regions. The mutation frequency of each region in each sample was calculated using the formula m = n/g, where n is the number of identified mutations and g is the number of generations. Accordingly, the mean mutation frequency of each treatment (five samples) was estimated by the ∑m/5. Numerical values above the bars indicate SNV rates in the genomic regions. The SNV rates of each genomic region (per site per generation) were estimated by dividing the number of observed mutations by the number of analyzed sites capable of producing a given mutation and the number of generations of MA