Fig. 4From: diffBUM-HMM: a robust statistical modeling approach for detecting RNA flexibility changes in high-throughput structure probing datadiffBUM-HMM has a very high specificity. A diffBUM-HMM only reports nucleotides with posterior probabilities of less than 0.4 on identically treated DMS-probed S. cerevisiae rRNA Structure-Seq (5.8S, 18S, 25S) and ChemModSeq 18S rRNA (18S#2) datasets. The box plot shows the distribution of the posterior probabilities for each rRNA sample. Shown are the posterior probabilities that the nucleotides were called modified in all replicates or differentially modified between replicates. B Base composition of nucleotides called modified in all replicates of the yeast 18S rRNA Structure-Seq data, when considering only nucleotides with posterior probabilities ≥0.95. C Nucleotides called modified in all replicates of the 18S rRNA Structure-Seq data (posterior probabilities ≥0.95) are highlighted in red in the secondary structure of the molecule. The names of the helices in the structure are indicated in blueBack to article page