Fig. 2

B cell and MEF reprogramming systems share a program of AS changes. A Schematic representation of MEF reprogramming time points analyzed by RNA-seq in [17]. B Percentage of exons in each B cell reprogramming AS cluster that are also detected as differentially spliced in the MEF reprogramming dataset of [17]. The number of events in each cluster is indicated at the bottom of the corresponding bar. The magenta dashed line indicates the average percentage for all 12 clusters. C PCA analysis of the 25% most variably expressed genes, segregated using k-means into 4 clusters: differentiated cells, early and late reprogramming and pluripotent cells, highlighted by colors and boxes. Circles: B cell reprogramming time points. Squares: MEF reprogramming time points. D Heatmap representing scaled PSI values (average between replicates) of exons differentially spliced in at least one time point in both B cell (left) and MEF reprogramming (right), with the corresponding hierarchical clustering. E Heatmap representing the expression of RNA-binding proteins known to be involved in pluripotency, somatic cell reprogramming, and/or development. Scaled cpm values (average between replicates) of both datasets are shown, with the corresponding hierarchical clustering. The bar plot (right) represents (when available) the fold change expression between ES cells and differentiated mouse tissues calculated in [7] and the corresponding ranking (color of the bar).