Skip to main content
Fig. 4 | Genome Biology

Fig. 4

From: Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf

Fig. 4

Distinct plastid and chloroplast growth phases, the plastid phase involving proliferation, genome replication and ribosome build-up. a Plastid number per cell. The region covered by coleoptile is shown. b Calculated plastid division rate. See Additional file 5: Table S6, S7 and S8 for details of microscopy calculations (n = 48 cells per sample) and cell proliferation corrections. c Expression (Z-score) of nuclear-encoded genes for plastid division-associated proteins. d Individual plastid/chloroplast size, measured for plastids positioned perpendicular to the direction of view (n = 10 plastids per cell). e Total plastid/chloroplast area per cell. f Chloroplast cellular growth rate, corrected for the effect of cell division, as in b. g Chloroplast index, ratio of the total chloroplast plan area in each cell and the plan area of the same cell. h As c, for proteins impacting the cellular chloroplast coverage. i Ratio of chloroplast genome (cpDNA) to haploid nuclear genome (gDNA) copies, measured in 3 biological replicates, each measured as 2 technical replicates, with the mean displayed. Error bars represent standard error of the mean between biological replicates. j As c, for plastid transcriptionally active chromosome proteins. k Ratio of chloroplast ribosomal RNA (cprRNA) to cytosolic ribosomal RNA (rRNA), quantified in each of four independent RNA preparations for each of the 15 samples. Values displayed as means ± standard error of the mean. l Expression, as c, for chloroplast translation-related proteins. m, n As c, for chloroplast protein import-associated translocon components. o Expression of genes for chloroplast transcription-related proteins. All individual genes are listed in Additional file 5: Table S4. The region of transition between the plastid and chloroplast growth phases, around sample 8 (identified in Fig. 4f), is shown as a gray band in every panel

Back to article page