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Fig. 3 | Genome Biology

Fig. 3

From: Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf

Fig. 3

Very early cell proliferation and subsequent cell growth coincide with expression of associated genes. a Summary of flow cytometric analysis of cell cycle stages for the first 14 samples. G1: gap 1. G2: gap 2. S: DNA synthesis phase. b Immunoblot of 20 μg of total protein from each of the first 14 samples, using antibodies specific to retinoblastoma-related protein 1 (RBR1) and its phosphorylated form (P-RB). The lower panel shows a replica gel stained for total protein with Coomassie brilliant blue. Molecular weights (kDa) are indicated on the right. c Expression of cell cycle-associated genes, displayed as Z-score. The individual genes are listed in Additional file 5: Table S4. d Box plots of cell size, measured as cell plan area, for each sample. See Additional file 5: Table S6 and Table S7 for details of microscopy calculations (n = 48 cells per sample) and for R script. The boxes represent upper and lower quartiles, with the middle line the median, and the whiskers the full range of observations. The fact that the leaf material for the first 8 samples was included within the seedling coleoptile is indicated. e Expression (Z-score) of cell wall synthesis-associated genes. f Expression (Z-score) of cell expansion genes

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