Fig. 5

Assessing false-positive and A>G edit detection for single cells using the nine normal tissue samples (N = 36,581 cells) with matched WGS data. a, c The per-cell FDR (a) and log10(A>G Edit Count). Each aligner/SNV caller is indicated as per the legend, and the horizontal lines above the plot indicate the results of a two-sided Wilcoxon signed-rank test (P < 0.001 blue otherwise red). b, d Representative comparison of the number of false-positive SNVs (b) and A>G Edits (d) versus the number of spliced + unspliced molecules in the cells from Eso_325_T0, the R² value and line of best fit as determined by ordinary least squares regression are indicated on the plot (P<0.001). The boxplots for a and c indicate the 25th, 50th, and 75th percentiles and the whiskers are 1.5 times the interquartile range