Fig. 2

The effect of sequencing saturation and the PCR duplicate rate on mRNA tag collapsing. a Collapsed molecule lengths from each sample ordered by their sequencing saturation (lowest to highest). For each sample, the collapsed read lengths were binned by their length and the resultant distributions were each down-sampled to 20,000 observations, smoothed using Gaussian kernel density estimation, and then scaled so the minimum and maximum value ranged from 0 to 1. The density values are indicated by the color bar. b The sequencing saturation (red) and PCR duplicate rates (blue) for each sample. c The percent of molecules in each of the collapsed base ranges as indicated by the legend