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Fig. 3 | Genome Biology

Fig. 3

From: Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immune regulatory pathways

Fig. 3

Approach for non-immune compartment profiling of metastatic liver and lung TME by RNAseq analysis. a KPC-bearing lung and liver are analyzed along with KPC cells from in vitro culture in 2D, normal lung, and normal liver as controls. All tissue samples are enzymatically dissociated into single cells. KPC-bearing liver and lung samples are further processed using negative magnetic bead selection to obtain the non-immune cells. b PCA plotting of the RNAseq dataset shows how individual samples cluster. c Expression of key genes identifying cells of pancreatic origin (KPC), tissue-specific parenchyma, and immune cells are shown in a heatmap to demonstrate the quality of the sample preparation process

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