Fig. 2

T cell profiling of metastatic liver and lung TME with CyTOF. a CD3+ subset of the dataset was re-clustered using both subtyping and functional markers. Select T cell clusters are represented with mean values for each group as radar plots (top). Separate radar plots with different axis ranges are used to facilitate the visualization of low-level subtypes. Relative values scaled for each immune cell subtype for all mice as a heatmap (bottom). Groups are annotated by color within the radar plot or as horizontal bars above the heatmap. FDR-adjusted p values compared using edgeR for KPC-bearing vs. normal conditions in the liver (LV_KpcNl) and lung (LG_KpcNl) as well as lung vs. liver in KPC-bearing (KPC_LgLv) and normal (NL_LgLv) conditions are annotated as an adjacent heatmap to the right. b Proportion of LAG3 positive CD8 T cells (top) and NK cells (bottom) as assessed by fluorescent flow cytometry comparing between KPC-bearing liver and lung is shown (n = 6). Two-tailed t test, ***p < 0.005. c Sample clonality values derived from TCRseq analysis of T cells within normal (gray) livers and lungs and KPC-bearing (red) livers and lungs are compared. Wilcoxon-Mann-Whitney, *p < 0.05; ***< 0.005