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Fig. 1 | Genome Biology

Fig. 1

From: Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immune regulatory pathways

Fig. 1

Global immune profiling of metastatic liver and lung TME with CyTOF. a Mice were injected with KrasG12D and TP53R172H-driven pancreatic cancer cells (KPC) into the liver by the hemispleen method or into the lung by intravenous tail vein injection. Normal lung and livers (day 0, “D0”) and day 21 (“D21”) from the day of injection were harvested and barcoded with a CD45 antibody conjugated to a unique metal. One sample from each group was then combined into a 4-plex batch to be stained with the full CyTOF panel (Table S1). b Heatmap of normalized marker expression for FlowSOM clustering of the dataset and c UMAP visualization of the clusters are shown. Two thousand events per sample are represented. Immune cell type proportions as a percentage of total CD45+ cells from each of the four groups are shown as d stacked barplots for the entire group and e mean values for each group as radar plots (left) and relative values scaled for each immune cell subtype for all mice as a heatmap (right). Groups are annotated by color within the radar plot or as horizontal bars above the heatmap. FDR-adjusted p values compared using edgeR for KPC-bearing vs. normal conditions in the liver (LV_KpcNl) and lung (LG_KpcNl) as well as lung vs. liver in KPC-bearing (KPC_LgLv) and normal (NL_LgLv) conditions are annotated as an adjacent heatmap to the right

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